Table 1.

Analysis of transposon derivatives selected by STMa

No.MutantIn vivo CIIn vitro CISimilarity to Y. pestis (%)Y. pestisORFSimilarity to NRDB (%)ProductMicroorganismPutative function
14H90.030.0697YPO005485 (256)Glycosyltransferase S. marcescens LPS core biosynthesis
22B31.020.8594YPO0337NSUK
35E4NTNT99YPO070246 (87)Putative lipoprotein Mycobacterium tuberculosis UK
43E10.480.3198YPO110891 (427)Citrate synthase S. enterica Citrate synthesis
52G80.531.3496YPO117439 (222)Adhesin E. coli UK
63C100.0551.0298YPO118666 (150)Hypothetical E. coli Amino acid transport
73H100.080.0797YPO1382 (promoter region)43LpsA, glycosyltransferase Pasteurella haemolytica LPS core biosynthesis
85G60.0210.8895YPO1987NSUK
91D120.0841.3497YPO1994NSUK
101H10NTNT98YPO217462 (442)UDP-glucose 6-dehydrogenase Rhizobium meliloti LPS/capsular polysaccharide biosynthesis
114H100.00361.9596YPO2287a65 (153)ABC transporter Methanococcus jannaschii Amino acid transport
125G70.251.0998YPO244082 (275)Iron(III) dicitrate ATP binding protein Haemophilus influenzae Iron transport
131A9NTNT100YPO2532NSUK
144H2NTNT98YPO271277 (218)RseA, negative regulator of RpoE (sigma 24) E. coli Transcription regulation of RpoE
155E60.271.2497YPO300447 (268)Prodipeptidase B. subtilis UK
161D20.430.0896YPO3099100 (468)ManC, mannose-1-P guanylyltransferase Y. pseudotuberculosis LPS biosynthesis
171D12-1NT0.1695YPO3099100 (468)ManC, mannose-1-phosphate guanyltransferase Y. pseudotuberculosis LPS biosynthesis
183G20.13NT98YPO3100100 (237)Fcl, fucose synthetase Y. pseudotuberculosis LPS biosynthesis
191B30.290.9890YPO3104100 (337)O-antigen polymerase Y. pseudotuberculosis LPS biosynthesis
201D9NT0.0798YPO3114100 (314)DdhB, CDP-D-glucose-dehydratase Y. pseudotuberculosis LPS biosynthesis
213F30.040.4495YPO3116100 (328)AscD, ascarylose biosynthesis Y. pseudotuberculosis LPS biosynthesis
223G10.211.2597YPO314489 (587)MdlB, multidrug resistance protein E. coli UK
235A50.441.1698YPO357288 (209)Hypothetical transcription factor E. coli Transcription regulation
241G6NTNT97YPO3657/8 (intergenic)NSUKUK
253F100.0170.4699YPO383487 (284)PldA, phospholipase A E. coli UK
261G6-1NT0.4196YPO396547 (397)VirA, His kinase Agrobacterium tumefaciens Sensory transduction
275D120.0030.98NS80 (66)Wzx S. enterica O-antigen transporter
285H10NTNTNS70 (30)Phage-related transcription activator Xylella fastidiosa Transcription activation
295B120.891.32NS59 (98)HypotheticalPhage HP1UK
301A9-1NTNTNSNSUKUK
311C9NT0.36NSNSUKUK
  • a Analysis of Y. pseudotuberculosis transposon derivatives selected by STM. NS, no similarity; UK, unknown; NT, not tested; Similarity to Y. pestis determined using BLASTn athttp://www.sanger.ac.uk/Projects/Y_ pestis; similarity of the corresponding Y. pestis gene product (length in amino acids shown parentheses) to an orthologue in the NRDB using BLASTp athttp://www.blast.genome.ad.jp.