Table 3.

Relative antigen 85B mRNA concentration in expression systems containing synthetic or wild-type antigen 85B genes

Time (min)a85B mRNA concnbSynthetic/wild-type ratio
Wild typeSynthetic
01.0c0.80.8
305.112.72.5
606.212.22.0
3007.012.11.7
  • a Minutes postinduction of expression vector with IPTG.

  • b Based on densitometry measurements.

  • c Value set at 1.