Table 1.

Titers of IgG1 and IgG2a antibodies in chronicallyT. gondii-infected mice and DNA-vaccinated mice against GRA1, GRA7, and ROP2 from T. gondii and Ag85A from M. tuberculosis

GroupNo.Titera
T. gondiiM. tuberculosis Ag85A
GRA1GRA7ROP2
IgG2aIgG1IgG2a/IgG1IgG2aIgG1IgG2a/ IgG1IgG2aIgG1IgG2a/IgG1IgG2aIgG1IgG2a/IgG1
C3Hb
 Infected12,8001,6008409,6006,400648000
 Vaccinated1409,6006,4006425,6006,40043,20010032
2409,60012,8003251,20080064160010016
3102,40025,60046,40008002004
4102,400102,40013,20012,8001/44000
5409,60025,60016102,4008001284000
BALB/c
 Infected1,6003,200 1/2204,8001,6001288005016
 Vaccinatedc 1204,800>819,200<1/4102,400204,8001/21,6004004
2204,80025,600825,60025,60018003,2001/4
3204,800102,40026,4003,20023,2003,2001
451,20051,200112,80012,800125,60080032
525,60025,60016,4001,600425,60080032
 Vaccinatedd
1<50800<1/16
2400>51,200<1/128
38006,4001/8
480025,6001/32
540025,6001/64
  • a Samples were considered positive at a given dilution if the OD exceeded 0.3.

  • b Pool from three animals.

  • c Individual animals vaccinated by intramuscular injection.

  • d Individual animals vaccinated using plasmid-coated gold particles and a gene gun.