Table 1.

PCR primers used for Intimin gene typing

PrimerPositionaOrientationSequencePCR product size with SK1 (bp)
SK126–46Forward5′-CCCGAATTCGGCACAAGCATAAGCNAb
SK2879–903Reverse5′-CCCGGATCCGTCTCGCCAGTATTCG881
LP22803–2829Reverse5′-CCCGAATTCTTATTTTACACAAGTGGC2,807
LP32788–2814Reverse5′-CCCGAATTCTTATTCTACACAAACCGC2,792
LP42283–2309Reverse5′-CCCGTGATACCAGTACCAATTACGGTC2,287
LP52605–2630Reverse5′-AGCTCACTCGTAGATGACGGCAAGCG2,608
  • a The position of LP2 primer is referred to as the eae sequence of human EPEC O127:H6 strain E2348/69 (M58154 ); the position of LP3 primer is referred to as theeae sequence of human EHEC O157:H7 strain EDL933 (Z11541 ); the position of LP4 primer is referred to as the eaesequence of rabbit EPEC O15:H− strain RDEC-1 (U60002 ); the position of LP5 primer is referred to as the eae sequence of O103:H2 strain PMK5 (AF116899 ).

  • b NA, not applicable.