Table 1.

Cytokine analysisa of culture supernatants of murine lymph node cells after in vitro stimulation with PfLSA-1-derived synthetic peptides

PeptidePeptide concn (μg/ml)Secretion of indicated cytokine (pg/ml) by:
BALB/c miceC57BL/6 mice
IL-2IFN-γIL-4IL-2IFN-γIL-4
LS1.10<575<540<10<5
10120180<53609,800<5
20120360<52605,600<5
402601,500<52801,200<5
LS1.2040100<58060<5
10160800<540010,400<5
20180660<53608,800<5
4090160<54409,200<5
LS1.3070120<515<10<5
10360600<518135<5
201803,000<530540<5
4013510,200<530675<5
LS1.4050120<58<10<5
10180450<5181,440<5
20165600<530600<5
401351,560<58510<5
LS1.5060<10<510<10<5
10165120<515<10<5
20225180<51845<5
4016545<51530<5
LS1.60<5<10<5<5<10<5
10<5<10<5<5<10<5
20<5<10<5<5<10<5
40<5<10<5<5<10<5
LS1.70<5<10<5<5<10<5
10<5<10<5<5<10<5
20<5<10<5<5<10<5
40<5<10<5<5<10<5
LS1R0<5<10<5<5<10<5
104552575<5<10<5
206070585<5<10<5
40135840240<5<10<5
  • a Cytokine analysis was performed by using specific murine cytokine ELISA kits. Data analysis was done with the respective standard cytokine curves.