Table 1.

Percentages of infected and apoptotic cells in resting orB. suis-infected cultures of human monocytesa

MonocytesMOI%b of:
Infected cellsApoptotic cells
RestingNAc 35 ± 4
B. suisinfected104 ± 15.6 ± 2.5
207.9 ± 2.45.8 ± 1.7
20015.3 ± 3.35.1 ± 2.1
OpsonizedB. suis infected2042 ± 136.5 ± 2.2
  • a Monocytes were infected (or not) with GFP-B. suis or opsonized GFP-B. suis in eight-chamber culture slides and cultured for 2 days before the percentage of infected cells was determined by microscopy, as described in Materials and Methods. Parallel experiments were performed to quantify the percentage of apoptotic cells by microscopy with fluorescein-labeled annexin V. Infections were performed with MOIs ranging from 1 to 200.

  • b Data are means and standard deviations from three different experiments.

  • c NA, not applicable.