Table 3.

Numbers of leukocytes present in brains of naive mice and control and immune mice on days 3 and 7 after infection withC. neoformans

CellsNo. of leukocytes per brain of the following mice on the indicated daya:
Naivei.c. infected
Saline-IFA treatedCneF-IFA treated
3737
Neutrophils512044,908138121,992
CD4+lymphocytes1,7601,240110,5262,645365,364
CD8+lymphocytes54842019,07578257,120
Macrophages2,8823,280300,2959,246537,540
Microglial16,95839,60064,20127,39395,064
  • a Pooled leukocytes isolated from the brains of naive or treated mice were counted and stained for flow cytometric analysis. To obtain the total number of each cell type present in each brain, the total number of leukocytes per mouse from each group was multiplied by the percentage of cells staining positive for the cell marker. Leukocytes were pooled from 10 mice per group. The experiment was performed twice, with similar results.