Table 1.

First validation set strains for F PCR assay

F serotype(s)F PCR resultStrain nameO:K:H serotype (or source strain, if clone)Source or reference
PutativeaConfirmatory
F7-1, F7-2NDb F7-1, F7-2AD110O6:K2:H1 18
F7-1d NDF7-1MC400/pDAL201B(AD110) 55
F7-2d NDF7-2HB101/pDAL210B(AD110) 55
F7-1, F7-2NDF7-1, F7-2CFT073O6:K2:H1 60
F7-1NDF7-1DH5α/pKgg201(CFT073) 60
F7-2NDF7-2DH5α/pDIW101(CFT073) 60
F7-2NDF7-2C952-79O6:K2:H1IEKC
F7-2NDF7-2C953-79O6:K2:H1IEKC
F7-2NDF7-2C1898-79O6:K2:H1IEKC
F7-2NDF7-22H4O6:K2:H1 41
F8F8, F10F8, F10C1253-77O18ac:K−:H−IEKC
F8F8F8, F10C1254-77O75:K−:H5IEKC
F8F8, F10F8, F10C351-82O18ac:K−:H−IEKC
F8F8, F10F8, F10C405-82O18ac:K−:H5IEKC
F8F8, F10e F8, F10C805-83O18ac:K−:H−IEKC
F8F F10C659-81O1:K51:H−IEKC
F8F8F8, F10C321-82O18ac:K−:H−IEKC
F8F8F8, F10C825-83O75:K5:H−IEKC
F8F8, F12F8, F12V30bO16:K1:H− 41
F9F9F9, F103669O2:K5:H4 12
F9d NDF9HB101/pDAL200A(3669) 12
F9F9F9, F10C1018-79O2:K5:H4IEKC
F9F9F9, F10C481-82O1:K1:H−IEKC
F9F9, F10e F8, F9C483-82O1?:K1:H−IEKC
F9F14, F16F13, F14, F16C878-83O4:K12:H5IEKC
F10NDF10C1960-79O7:K1:[H1]IEKC
F10NDF10C997-79O7:K1:H−IEKC
F10NDF10C328-82O7:K1:H−IEKC
F10NDF10C906-83O7:K1:H−IEKC
NAc F F10G1062aO4:K7:HR 38
F11F11, F16e F11, F16IA2O4:K12:H− 10
F11NDF11HB101/pDC1(IA2) 16
F11NDF11C1976-79O1:K1:H7IEKC
F11NDF11C974-79O1:K1:H7IEKC
F11F10e, F11F10, F11V31O4:K12:H− 41
F11, F16NDF11, F16AFR015O4:K+:H− 45
F11, F16F10e, F11, F16F10, F11, F16BOS040O4:K+:H1 45
NAF10, F11, F16F10, F11, F16JR1O4:K7:H1 82
F12-1, F12-2NDF12C1979-79O16K1:H−IEKC
F12-2NDF12C493-82O6:K13:H1IEKC
F12-2NDF12C438-82O75:K5:H5IEKC
F12-2NDF12C469-82O75:K95:H5IEKC
F13NDF13J96O4:K−:H5 44
F13NDF13DH5α/pJJ48(J96: allele I) 47
F13NDF13HB101/pJFK102(J96: allele III) 51
F13F13, F14F13, F14CP9O4:K10,K54/96:H5 44
NANDF13NM554/pCP9I(CP9)This study
NANDF14NM554/pCP9III(CP9)This study
F13F13, F14F13, F14BF1023O4:K10,K54/96:H5 44
F13F13, F14F13, F14BF1056O4:K10,K54/96:H5 44
F13F13, F14F13, F14BOS038O4:K10,K54/96:H5 44
F13F13, F14F13, F14BOS110O4:K10,K54/96:H5 44
F13F13, F14F13, F14518O4:K10,K54/96:H5 45
F13NDF13, F1418878O4:K10,K54/96:H5IEKC
F13NDF13BF1040O4:K3:H5 44
F13NDF13BF9043O4:K3:H5 44
F13NDF13CA002O4:K3:H5 45
F13NDF13CA062O4:K3:H5 45
F13NDF9, F13CA022O4:K3:H5 45
F13F11,e F13F11, F13R28O4:K3:H5 45
F13F13, F16F13, F163048O4:K−:H− 31
F13, 16NDF13, 14, 16C134-73O4:K12:H5IEKC
F13, F14, F16NDF13, F14, F1620025O4:K12:H−IEKC
F14NDF14C127-86f (Unknown)IEKC
F14NDF14C 818-83O25:H9IEKC
F14NDF14C1023-79O83:K24:H31IEKC
F15NDF10, F15C1805-79O75:K5:H−IEKC
F15F F10C826-83O75:K5:H−IEKC
F15F10e, F15F10, F15C372-82O75:K5:H+IEKC
F15F10, F15F10, F15C394-82O75:K5:H−IEKC
F15F10e, F15F10, F15C312-82O75:K5:H−IEKC
F16NDF16C83-83O157:K−:H45IEKC
F16F14, F16F14, F16PM8O4:K12:H− 41
F16F16F7-1, F16BOS021O4:K+:H− 45
F16NDF16BOS046O4:K12:H− 45
  • a According to records of the IEKC.

  • b ND, not done.

  • c NA, not applicable.

  • d F type according to reference12.

  • e Antigen detected only in second or third round of confirmatory serotyping.

  • f Recombinant.