Table 1.

vlsE plasmid hybridization and PCR results in clinical isolates of B. burgdorferi

IsolateaPassage no.Size (kb) of plasmid hybridizing with vlsEprobebvlsE PCR resultcRFLP typed
B14136+1
B161282
B231323
B57232+1
B247428+2
B2822252
B2872282
B294232+1, 2
B296228+1
B305228+1
B310232+1
B3112282
B3221323
B327221+3
B3291282
B3323322
B3332323
BL362383
BL1462282
BL152228+1
BL1544282
CD160228+1
  • a Isolates with a B or CD designation were cultured from EM lesion biopsies, and those with a BL designation were cultivated from blood.

  • b Molecular size of plasmid to which B31-5A3vlsE probe hybridized, as determined by Southern blot analysis of PFGE-separated plasmids.

  • c Presence (+) or absence (−) of a band detectable by ethidium bromide staining following PCR amplification using B31 vlsE-based primers F4120 and R4066.

  • d Based on PCR-RFLP analysis of the 16S-23S rDNA spacer (8).