Table 1.

Cellular features observed during timed coculture ofB. pseudomallei and A. astronyxis

Time (min)Adherent bacillibNo. of trophozoitesa
Rotating bacillicSingle-bacillus vacuolesdMultibacillary vacuoleseBacillary tuftsfBacillary tanglesg
04.3 ± 1.51.7 ± 0.60.3 ± 0.60.0 ± 0.00.0 ± 0.00.0 ± 0.0
2012.3 ± 3.86.0 ± 2.61.7 ± 1.52.0 ± 3.50.0 ± 0.00.0 ± 0.0
4013.7 ± 3.06.0 ± 3.03.7 ± 2.15.7 ± 5.10.3 ± 0.60.0 ± 0.0
6012.0 ± 2.63.0 ± 2.04.0 ± 1.710.3 ± 3.51.0 ± 1.00.0 ± 0.0
1,44020.0 ± 0.017.7 ± 3.23.3 ± 1.512.3 ± 0.616.3 ± 1.50.7 ± 0.6
  • a At each time interval, 20 trophozoites were examined for the presence of each indicated feature. Values shown are the mean ± standard deviation of the number of trophozoites exhibiting each feature from three consecutive replicate experiments.

  • b Bacilli adherent to external trophozoite surface.

  • c One or more adherent bacilli in high-frequency rotation around point on external trophozoite surface.

  • d One or more trophozoite vacuoles containing single bacilli.

  • e One or more trophozoite vacuoles containing more than one bacillus.

  • f Five or more bacilli in single tuft on external trophozoite surface.

  • g Bacilli in tangled web of chains external to trophozoite or cyst surface.