Table 1.

Examples of sPCR probes hybridized to pathotype 5 isolates

Pathotype 5 virulence signaturea and groupHybridization [no. (%)] to probe:
P5P10P13′P24′P37P64P68P69
1000110000000
 UTI (n = 23)14 (61)13 (57)18 (78)21 (91)1 (4)1 (4)2 (9)7 (30)
 Fecal (n = 27)18 (67)17 (63)23 (85)20 (74)2 (7)1 (4)2 (7)6 (22)
1001110000000b
 UTI (n = 15)11 (73)11 (73)13 (87)13 (87)8 (53)13 (87)12 (80)4 (27)
 Fecal (n = 30)19 (63)16 (53)20 (67)21 (70)13 (43)24 (80)20 (67)4 (13)
1100110000100
 UTI (n = 13)13 (100)12 (92)12 (92)13 (100)0 (0)1 (8)3 (23)1 (8)
 Fecal (n = 19)18 (95)18 (95)19 (100)15 (79)1 (5)0 (0)1 (5)3 (16)
1101110000100
 UTI (n = 25)21 (84)9 (36)8 (32)12 (48)20 (80)19 (76)18 (72)16 (64)
 Fecal (n = 8)5 (63)1 (13)2 (25)4 (50)5 (63)5 (63)6 (75)3 (38)
All other signatures
 UTI (n = 18)11 (61)10 (56)14 (78)15 (83)4 (22)9 (50)7 (39)6 (33)
 Fecal (n = 10)6 (60)4 (40)6 (60)5 (50)2 (20)5 (50)3 (30)3 (30)
Total pathotype 5
 UTI (n = 94)70 (74)55 (59)65 (69)74 (79)33 (35)43 (46)42 (45)34 (36)
 Fecal (n = 94)66 (70)56 (60)70 (74)65 (69)23 (24)35 (37)32 (34)19 (20)
Probe locationc 1111110241 and 42
  • a Hierarchically defined groups based on the associations among all genes. Those with the strongest associations combined with known biologic evidence for physical linkage were classified as a pathotype (28). 1 and 0, presence and absence, respectively, of the following genes (in order):fim, prf, sfa, aer,kpsMT, ompT, hly, cnfl,drb, capIII, papGad,prsGj96, and papGj96.

  • b Virulence signature of tester and driver strains.

  • c PFGE band of tester (366-11) DNA cleaved withNotI and hybridized.