Table 3.

Secretion phenotypes of wild-type and mutant V. cholerae O1 El Tor rugose strains

StrainaAntibiotic resistancebNo. of TnphoAinsertionsMap location of TnphoA insertion(s) (kb of SfiI fragment)cHemolysisdMotilityeCT activity (% of total) in:
MediumCells
S1KanrAmpr 1677++++++991
S2KanrAmpr 1145.5+++++991
S3Kanr Ampr 2291, 339++8020
S4KanrAmpr 2291, 339, 3886634
S7KanrAmpr 1291++++++991
S8KanrAmpr 1677++++++991
S10KanrAmpr 1165++++991
S11Kanr Ampr 2291, 490++++++7030
S13KanrAmpr 2291, 490+++++991
S14Kanr Ampr 3291, 490, 525+++++991
S16KanrAmpr 2291, 280++++++5347
S17KanrAmpr 1291++++++8614
S18Kanr Ampr 2 80, 425++++++7525
S20KanrAmpr 1291++++++991
NS1KanrAmpr 12912872
NS25KanrAmpr 1435++++++NDf ND
N16961 RKanrAmpr None++++++991
AA16KanrAmpr None++++++982
AA10Kanr None+3664
AA10/pDSK-2KanrAmpr None++++991
AA1Kanr None8713
AA1/pAA44KanrAmpr None+++991
  • a Strains S1 to S20 were derived from C6706 rugose; NS1 and NS25 were derived from N16961 rugose

  • b Ampr is probably due to cointegration of the transposon delivery vector

  • c In PFGE analysis

  • d Hemolytic activity on 5% rabbit blood agar plates after 24 h of incubation at 37°C. +++, ++, +, and −, complete, moderate, weak, and no lysis, respectively.

  • e Motility determined after 4 to 6 h of incubation at 37°C on motility agar. +++, ++, +, and −, high, moderate, weak, and nonmotile phenotypes, respectively.

  • f ND, not determined.