Table 1.

Primer sequences and annealing temperatures

PrimerSequenceAnnealing temp (°C)
hBD-2 (5′) CCA GCC ATC AGC CAT GAG GGT 65
hBD-2 (3′) GGA GCC CTT TCT GAA TCC GCA
IL-8 (5′) TTT CTG ATG GAA GAG AGC TCT GTC TGG 60
IL-8 (3′) AGT GGA ACA AGG ACT TGT GAA TCC TGG
TNF-α (5′) TTC TGC CTG CTG CAC TTT GGA CTC AT 60
TNF-α (3′) TTG ATG GCA GAG AGG AGG TTG ACC TT
MCP-1 (5′) CTG CTC ATA GCA GCC ACC TTC ATT 54
MCP-1 (3′) GCA CAG ATC TCC TTG GCC ACA ATG
RPO (5′) AGC AGG TGT TCG ACA ATG GCA 50
RPO (3′) ACT CTT CTT TGG CTT CAA CC
Keratin (5′) GTC CTC TCC ATG GAC AAC AAC 49
Keratin (3′) TGT CAA TCT CGG CTC TCA GCC