Table 1.

Oligonucleotide primer sequences used for PCR

OligonucleotideSequencea
Degenerate primers
 LVIEDCCC CTC TAG ACT NGT NAT NGA NGA
 GADDYCCC CGC ATC CGT AAT CAT CNG CGC C
DNA probes (mapping)
 HI0401 (omp1)TCG TTG CGC CAG TGA ATG ATA A
GCC CCT AAT GCA ACA CGA GAG T
 HI0406 (accA)ATC GCC CAC GCC AAT AGC
ATC GGT CAT CAA AAA GGT CGT TCT
 HI0410 (tyrR)GTG CGG GTT TGC CTG ATG
ACG AAC AAG CGC GAT AAA GAG T
 HI0429 (glmS)TGC CGC CAT ATA GCC CTT TTC
GTG TTA CCC GCC GTT TTA TCT TTT
 HI0444 (topB)AGC GGA TGT GGC AAG AGG AAT AAA
TGC GGC GAT AAT GAT GTG GTA ACT
 HI0445 (secG)CAT CAG GTA CAA TGT TTG GCT CTG
TGT CTT TCG CTG GAG CTG CTT
 HI0446 (fruA)CCC GCA TCG CAT TGG CTA AC
TAA TGC GGG AAC GAA AGA AGA AAG
 HI0448 (fruB)GCC CCG CAT TAA TCG CAA CTA
ATG GCA TCG CTA TTC CTC ACG
 HI0457 (pabC)TTC TTG CAC CGC TTT GTT ATG TT
TTG GCG AAA AGA TCT TGA AAA TG
 HI0465 (serA)AAT AAA TCC GCG ACT GGC TCT CA
CGG GCT CAA CGG GGA ATA CA
DNA probes (screening)
 Region ICGG TAA ATG CGG AAT GGT CA
GCC ACT CTT TGA CAA ATG GTT GAG
 Region IIGTG CCA CCT TTC TAA TTG TTG CTG
GAA CGA TAG CAC GCC TTT TAA CC
 Region IIITTC GCT TGT TCT CTC CAC GC
GAC CGC ACT TTT TAC CTT TGT CA
Long PCR primers
 F5ECTT TGA CTT GTG CGC AAT AAG TCG
TAA TGC GGG AAC GAA AGA AGA AAG
 F10GCAT CAG GTA CAA TGT TTG GCT CTG
GCA TTA CGC AGC TTT CGT ATC GT
  • a Underlined region in LVIED isXbaI site; underlined region in GADDY is BamHI site.