Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotypeReference or source
E. coli strains
 HB101F′laqI q 2
 KB53 fimH gene from wild-type strain CI#4 33
 KB59 fimH gene from wild-type strain CI#3 33
 KB91 fimH gene from wild-type strain F-18 33
 KB96 fimH gene from wild-type strain MJ2-2 33
 S1918F′laqI qΔfimB-H::kan 3
 MS72pMAS1 and pPKL115 in S1918This study
 MS205pMAS63 and pPKL115 in S1918This study
 MS206pMAS64 and pPKL115 in S1918This study
 MS208pMAS66 and pPKL115 in S1918This study
 MS229pMAS70 and pPKL115 in S1918This study
 MS233pMAS72 and pPKL115 in S1918This study
 MS237pMAS74 and pPKL115 in S1918This study
 MS239pMAS75 and pPKL115 in S1918This study
 MS243pMAS77 and pPKL115 in S1918This study
 MS272pMAS88 and pPKL115 in S1918This study
 MS273pMAS89 and pPKL115 in S1918This study
 MS276pMAS92 and pPKL115 in S1918This study
 MS278pMAS94 and pPKL115 in S1918This study
 MS279pMAS95 and pPKL115 in S1918This study
Plasmids
 pPKL4Wild-type fim gene cluster 14
 pPKL115All fimgenes except fimH 23
 pMAS1 fimH 28
 pMAS63Modified fimH gene (L125F, F142Y)This study
 pMAS64Modified fimH gene (G73E, L107F)This study
 pMAS66Modified fimH gene (Y175F, A204E)This study
 pMAS70Modified fimHgene (P490, P180Q)This study
 pMAS72ModifiedfimH gene (A188E)This study
 pMAS74ModifiedfimH gene (I52T, N152Y, G159V)This study
 pMAS75Modified fimH gene (A25P, R92P, D141N)This study
 pMAS77Modified fimH gene (L68F, S114R)This study
 pMAS88Modified fimHgene (A18T, F43S, R132Q, F215Y)This study
 pMAS89ModifiedfimH gene (R92H)This study
 pMAS92ModifiedfimH gene (S17G, K101E, Y175H, S216P)This study
 pMAS94Modified fimH gene (L183H, H201N)This study
 pMAS95Modified fimH gene (H201Q)This study
 pPKL229Modified fimH gene (A25P, R92P)This study
 pPKL230Modified fimH gene (D141N)This study
 pPKL231Modified fimH gene (A25P)This study
 pPKL232Modified fimH gene (R92P, D141N)This study
 pTBK31Modified fimHgene (G73E)This study
 pTBK32Modified fimH gene (L107F)This study
 pTBK35Modified fimH gene (P49Q)This study
 pTBK36Modified fimH gene (P180Q)This study
 pTBK37Modified fimH gene (L68F)This study
 pTBK38Modified fimH gene (S114R)This study