Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or phenotypeSource or reference
Strain
 OG1RFGelatinase and serine protease production positive/(Gel+, Spr+) 10
 TX5128 gelE transposon mini-γδ insertion mutant of OG1RF, Gel, Spr, Kanr 19
 TX5240 fsrA insertion mutant of OG1RF, Gel, Spr, Kanr This study
 TX5241 fsrB insertion mutant of OG1RF, Gel, Spr, Kanr This study
 TX5242 fsrC insertion mutant of OG1RF, Gel, Spr, Kanr This study
 TX5243 sprE insertion mutant of OG1RF, Gel+, Spr, Kanr This study
 TX5248 orf1 insertion mutant of OG1RF, Gel+, Spr+, Kanr This study
 TX5244TX5240 harboring plasmid TEX5249, Gel+, Spr+, Kanr Emr This study
 TX5245TX5241 harboring plasmid TEX5249, Gel+, Spr+, Kanr Emr This study
 TX5246TX5242 harboring plasmid TEX5249, Gel+, Spr+, Kanr Emr This study
 TX5247TX5128 harboring plasmid TEX5249, Gel, Spr, Kanr Emr This study
 DH5α E. coli host strain for routine cloningStratagene
 TX5249DH5α(pTEX5249)This study
Plasmids
 pBluescript SK(−)Cloning vector, Ampr Stratagene
 pTEX4577Suicide vector for single cross-over mutagenesis in E. faecalis, Kanr 19
 pAT18Shuttle vector for E. coli and E. faecalis, Emr 23
 pTEX5249pTEX5249 was constructed by cloning a 6-kb PstI/BglII fragment containing fsrA, fsrB, and fsrC into the shuttle vector pAT18, Emr This study