Table 1.

Enumeration of cytokine-producing cells following primaryC. trachomatis genital tract infectiona

Days postinfectioncNo. of cytokine-producing cells/10 fieldsb
IL-4IL-10IL-12TNF-α
30 0 0 0 
76.5 (0–19)4.2 (0–15)2 (0–4)16 (1–31)
1012 (7–16)19 (11–41)3 (2–4)2 (0–3)
14130 (104–213)254 (159–320)0.5 (0–1)3 (1–5)
21242 (102–388)340 (106–619)7 (2–13)21 (10–40)
28168 (128–247)303 (182–468)6 (3–10)18 (4–42)
35122 (114–133)234 (179–342)18 (5–36)29 (2–57)
42182 (84–270)257 (134–338)8 (6–16)38 (21–49)
7065 (41–85)69 (45–103)12 (2–16)42 (7–90)
  • a Uterine horns were removed from infected mice at various times following primary infection, and the tissue was processed as described in Materials and Methods for the detection of cytokine-producing cells.

  • b Cytokine-producing cells were enumerated by counting positive-staining cells in 10 microscopic fields using a 20× objective (total area evaluated, 9.5 mm2). Data are presented as the mean number of cytokine-producing cells per 10 fields from four or five mice per time point; the range is given in parentheses.

  • c Days following primary genital tract infection.