Table 3.

Properties of the C. jejuni 81116galE mutant virulence indicators compared to those of the parent wild-type strain

Strain% Adherencea% Invasiona% SurvivalbHemolysis (% of lysed sheep erythrocytes)% DNA uptakec
4 h17 h41 h
Wild type10010025.6 ± 12.37.7 ± 2.034.7 ± 22.261 ± 8.9100
galEmutant4.6 ± 5.20.9 ± 1.224.1 ± 14.38.5 ± 1.346.3 ± 7.286.9 ± 26.44.5 ± 5.1
  • a Adherence or invasion of INT407 cells by C. jejuni 81116 galE mutant compared to the parent strainC. jejuni 81116. The number of 81116 cells that adhered or invaded was taken as 100%.

  • b Percentage of surviving cells after incubation for 30 min with NHS.

  • c Transformation efficiency (calculated per microgram of DNA per 108 CFU) compared to the transformation efficiency of 81116.