Table 1.

Effect of phoP-constitutive alleles onpag transcriptional activity

Background strainRelevant genotypeRelevant phenotypePlasmidReporter activity (U)a
JSG208 pho24 pagB::MudJPhoPQ constitutive,pagB-lacZ NAb 321 ± 16 (β)
JSG465 pagB::MudJphoP::Tn10d-TetPhoP,pagB-lacZ pWSK200c 6.2 ± 2 (β)
JSG465 pagB::MudJphoP::Tn10d-TetPhoP,pagB-lacZ pPC3-2d 390 ± 14 (β)
JSG225 pagD::TnphoA phoP::Tn10d-Tet phoN2 zxx::6251Tn10d-CamPhoP,pagD-phoA pWSK2008.3 ± 2 (A)
JSG225 pagD::TnphoA phoP::Tn10d-Tet phoN2 zxx::6251Tn10d-CamPhoP,pagD-phoA pPC3-2784 ± 22 (A)
JSG225 pagD::TnphoA phoP::Tn10d-Tet phoN2 zxx::6251Tn10d-CamPhoP,pagD-phoA pPSK200-691e 124 ± 8 (A)
JSG225 pagD::TnphoA phoP::Tn10d-Tet phoN2 zxx::6251Tn10d-CamPhoP,pagD-phoA pPSK200-7974f 540 ± 10 (A)
JSG208 pho24 pagD::TnphoA phoN2 zxx::6251Tn10d-CamPhoPQ constitutive,pagD-phoA NA674 ± 19 (A)
  • a Units reported as described by Miller (22) for β-galactosidase (β) or alkaline phosphatase (A).

  • b NA, not applicable.

  • c pWSK29 with the wild-type phoPgene.

  • d pWSK29 with the double-mutant, constitutivephoP gene.

  • e pWSK29 with the single-mutant (784 A→G; Q203R), constitutive phoP gene.

  • f pWSK29 with the single-mutant (418 G→A; S93N), constitutive phoP gene.