Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype and/or phenotypeSource or reference
S. enterica serovar Typhimurium strains (plasmid)
 SR-11 gyr1816 4
 SR-11 Fad gyr1816 cra::Tn10 d cam 21
 SR-11 Fad AX-2 gyr1816 cra::Tn10 d cam This study
 SR-11 Fad(pBR322) gyr1816 cra::Tn10 d cam, bla tet This study
 SR-11 Fad(pJHA8) gyr1816 cra::Tn10 d cam, cra+tet This study
 LJ2443 fruR51::Tn10 3
 UK-1Virulent avian strain 5
 UK-1 Fad AX-1 cra::Tn10 d cam This study
E. coli S-17 λpir Host for pLD55 and pMJN10V. de Lorenzo
Plasmids
 pBR322 bla tet 2
 pJHA11.4-kb camgene from Tn10::d cam inBamHI site of pBR322This study
 pJHA74.3-kbPstI fragment containingcra::Tn10 d cam in pBR322, cam tet This study
 pJHA81.4-kbcra + gene in PstI site of pBR322,tet This study
 pLD55Suicide vector for allelic exchange, bla tetAR 15
 pMJN104.3-kb PstI fragment containingcra::Tn10 d cam in pLD55, cat bla tetAR This study