Table 2.

Effect of saccharides on the binding of F1C fimbriated recombinant E. coli HB101(pPIL110-54) to immobilized asialo-GM1 (GgO4Cer) or asialo-GM2 (GgO3Cer)

SaccharideInhibition (mean % of control)
Conc (mM)Asialo-GM1(GgO4Cer)Asialo-GM2 (GgO3Cer)
d-Galactose105357
11210
Galβ1-3GalNAc105137
186
Galβ1-4GlcNAc105141
1107
GalNAcβ1-4Gal-spacer-BSAa 0.266469
0.0264045
N-Acetyl-d-galactosamine104747
1129
Lactose104841
1118
  • a Molarity of disaccharide GalNAcβ1-4Gal linked to neoglycoprotein according to the company's information. Binding of nonfimbriated control HB101(pACYC 184) strain or fimbriated bacteria HB101(pPIL110-54) suspended in buffer only was used as a control value and was represented as 0.0 and 100%, respectively.