Table 1.

Glycolipids screened for the binding of purified F1C fimbriae on thin-layer chromatograms and F1C fimbriated recombinant bacteria on ELISA plates

NameaBindingStructure
TLCboverlayELISAc
CeramideCer
Galactosylceramide (GalCer1)±±Galβ1-1Cer (hydroxylated fatty acids)
Galactosylceramide (GalCer2)++Galβ1-1Cer (nonhydroxylated fatty acids)
Sulfatide (SFT)SO3Galβ1-1Cer
GalabiosylceramideNDGalα1-4Galβ1-1Cer
Glucosylceramide (GlcCer)++Glcβ1-1Cer
Lactosylceramide (LacCer)++++Galβ1-4Glcβ1-1Cer
Globotriaosylceramide (Gb3Cer)++++Galα1-4Galβ1-4Glcβ1-1Cer
Globotetraosylceramide (Gb4Cer)GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1Cer
Forssman antigen (Gb5Cer)NDGalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-4Glcβ1-1Cer
Disialosylparagloboside (DSPG)NDNeuα2-8NeuAcα2-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc1-1Cer
Disialosyllacto-N-nor-hexaosylceramide (DSnHC)NDNeuα2-8NeuAcα2-3(Galβ1-4GlcNAc)2β1-3Galβ1-4Glc1-1Cer
Paragloboside (nLc4Cer)++++Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer
Lactotriaosylceramide (Lc3Cer)++++GlcNAcβ1-3Galβ1-4Glcβ1-1Cer
Gangliotriaosylceramide (asialo-GM2, GgO3Cer)++++++++GalNAcβ1-4Galβ1-4Glcβ1-1Cer
Gangliotetraosylceramide (asialo-GM1, GgO4Cer)++++++Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1Cer
GM1 Galβ1-3GalNAcβ1-4(NeuAcα2-3)Galβ1-4Glcβ1-1Cer
GM2 GalNAcβ1-4(NeuAcα2-3)Galβ1-4Glcβ1-1Cer
GM3 NeuAcα2-3Galβ1-4Glcβ1-1Cer
GD2 GalNAcβ1-4Gal(3-2NeuAc8-2αNeuAc)β1-4Glcβ1-1Cer
GD3 NeuAcα2-8NeuAcα2-3Galβ1-4Glcβ1-1Cer
  • a Glycolipids are abbreviated according to the recommendations made in reference 15. Purified glycolipids were obtained from Sigma.

  • b Binding to glycolipids separated on HPTLC is graded as follows: ++++, optimal binding; +++, suboptimal binding; ++, strong binding; +, weak binding; ±, very weak binding; −, no binding.

  • c Binding to glycolipids immobilized on ELISA plates is graded as follows: ++++, absorbance of >3.00 at 450 nm; +++, absorbance of >2.00 at 450 nm; ++, absorbance of >1.00 at 450 nm; +, absorbance of >0.5 at 450 nm; ±, absorbance of >0.2 at 450 nm; and ND, not done.