Table 1.

Amino acid sequences of synthetic overlapping peptides of ESAT-6 and CFP-10 used in this study

Peptide (position)Amino acid sequencea
ESAT-6
 p1 (1–20)MTEQQWNFAGIEAAASAIQG
 p2 (11–30)IEAAASAIQGNVTSIHSLLD
 p3 (21–40)NVTSIHSLLDEGKQSLTKLA
 p4 (31–50)EGKQSLTKLAAAWGGSGSEA
 p5 (41–60)AAWGGSGSEAYQGVQQKWDA
 p6 (51–70)YQGVQQKWDATATELNNALQ
 p7 (61–80)TATELNNALQNLARTISEAG
 p8 (71–90)NLARTISEAGQAMASTEGNV
 p9 (76–95)bISEAGQAMASTEGNVTGMFA
CFP-10
 p1 (1–20)MAEMKTDAATLAQEAGNFER
 p2 (11–30)LAQEAGNFERISGDLKTQID
 p3 (21–40)ISGDLKTQIDQVESTAGSLQ
 p4 (31–50)QVESTAGSLQGQWRGAAGTA
 p5 (41–60)GQWRGAAGTAAQAAVVRFQE
 p6 (51–70)AQAAVVRFQEAANKQKQELD
 p7 (61–80)AANKQKQELDEISTNIRQAG
 p8 (71–90)EISTNIRQAGVQYSRADEEQ
 p9 (81–100)VQYSRADEEQQQALSSQMGF
  • a From the N terminus to the C terminus.

  • b Peptide 9 of ESAT-6 overlaps peptide 8 by 15 aa.