Table 1.

Hydrogen peroxide sensitivity and production by various bacterial pathogens

SpeciesMIC (mM)aMBC (mM)bH2O2 generated (mM)cSource or referenced
Gram-negative
H. influenzaeRd0.40.5<0.1 26
H. influenzaeEagan0.40.5<0.1 26
N. meningitidis MC58C30.45<0.1 32
M. catarrhalis Bc11.1160<0.1Clinical isolate
E. coliRS218ND15<0.1 1
S. enterica serovar Typhimurium LT2ND20<0.1Collection of K. Sanderson
K. pneumoniae Kp1ND20<0.1Clinical isolate
P. aeruginosa PA01ND60<0.1ATCC 15692
Gram-positive
S. pyogenesP87ND40<0.1Clinical isolate
S. agalactiae P60ND80<0.1Clinical isolate
S. equisimilis P107ND20<0.1Clinical isolate
E. faeciumP119ND80<0.1Clinical isolate
S. aureus A1ND10<0.1Clinical isolate
S. pneumoniae strains
 P394 (type 4)1.680 TIGR genome strain
 D39 (type 2)1.2800.44 ± 0.08 4
 P383 (type 6B)NDND0.53 ± 0.08 22
 P384 (type 6A)NDND0.71 ± 0.13 22
 P878 D39 (spxB::TnphoA)1.680<0.1 38
 P62 (type 9V opaque variant)NDND<0.1 22
 P64 (type 9V transparent variant)NDND0.43 ± 0.13 22
  • a The MIC was determined as the minimum concentration of H2O2 necessary to prevent turbid growth of a 1-in-50 inoculum of a stationary-phase culture following overnight incubation at 37°C. ND, not determined.

  • b The MBC was determined as the minimum concentration of H2O2 necessary for >99.9% killing of washed, log-phase cells in BHI medium after 30 min at 37°C.

  • c H2O2 concentration present in culture supernatants after incubating approximately 5 × 107 washed, log-phase cells for 1 h in BHI medium at 37°C.

  • d TIGR, The Institute for Genome Research.