Table 4.

Results of PCR and restriction analysis of Stx1-positiveE. coli strains used in this study with specific primersa

StrainSerotypestx genotypecPCR resultb with primer:Sizes (bp) ofPvuII fragments of stxB1-f/s-stx-b
Q-stx-f/285 (700 bp)stxB1-f/85a-stx1-b (868 bp)stxB1-f/282-stx1-b (1,871 bp)stxB1-f/s-stx-b (3,016 bp)
EDL933O157:H71, 2++++560, 1,200, 1,300
A8993CS2O157:H71, 2++++560, 1,200, 1,300
HUSCL8O157:H71, 2++++560, 1,200, 1,300
RO10O26:H111++++560, 1,200, 1,300
1858/96O103:H21++++1,000, 2,000
2905/96O103:H21++++1,000, 2,000
3942/96O103:H21++++1,000, 2,000
PMK3O103:H21++++1,000, 2,000
UTIO103:H21++++1,000, 2,000
TB-325AO26:H 1++++1,000, 2,000
ED-17O26:H 1++++1,000, 2,000
5080/97O26:H 1, 2++++1,000, 2,000
TB334O26:H 1++++1,000, 2,000
SK004O26:H 1++++1,000, 2,000
5236/96O26:H111++++1,000, 2,000
1639/97O111:H 1+
ED-31O111:H 1+
78/92O111:H 1+
SK003O111:H 1, 2+
C600(933W)  2
  • a For primer description see Table 2.

  • b PCR results: +, product obtained; −, no product obtained.

  • c 1, stx 1; 2,stx 2.