Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
Bacterial strains
V. vulnificus
  ATCC 29307Clinical isolate; virulentLaboratory collection
  MO6-24/OClinical isolate; virulent 55
  KC64ATCC 29307vvpE::pKC9844; elastase deficientThis study
  CMM111MO6-24/O vvpE::pKC9844; elastase deficientThis study
E. coli
  DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Laboratory collection
  SY327λpir Δ(lac pro)argE(Am) recA56 gyrA rpoB λpir; host for π-requiring plasmids 21
  SM10λpir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir; Kmr; host for π-requiring plasmids; conjugal donor 21
Plasmids
 pNQ705Cloning vector; R6K γ ori (requires π);oriT of RP4; Cmr 22
 pRK415IncP ori; broad-host-range vector;oriT of RP4; Tcr 13
 pKC980pUC18 with 2.5-kb partial Sau3A fragment encoding vvpE; Apr This study
 pKC9844pNQ705 with 0.6-kbHindIII-PstI fragment encoding internal coding sequence of vvpE; for allelic exchange; Cmr This study
 pRKY980pRK415 with 2.5-kbEcoRI-SalI fragment carrying vvpE from pKC980; Tcr This study
  • a Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Tcr, tetracycline resistant.