Table 1.

SPE A mutants used in this work and plasmids carrying them

MutantAmino acid change(s)Plasmid(s)Reference
Vector onlyNonepMIN164 5
Wild-type SPE ANonepMIN165 5
N20DAsn→AsppMIN166 29
D45NAsp→AsnpMIN170This work
C98SCys→SerpMIN173 29
N20D/C98SAsn→Asp, Cys→SerpMIN167 29
N20D/D45N/C98SAsn→Asp, Asp→Asn, Cys→SerpMIN169, pLP613This work
Q19H/N20D/L41A/L42A/D45N/C98SGln→His, Asn→Asp, Leu→Ala, Leu→Ala, Asp→Asn, Cys→SerpLP635This work