Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSource or reference
E. faecalis
  OG1RFAdh+Fusr Rifr 18,39
  TX5256OG1RFace::pTEX5253; ace insertion disruption mutant of OG1RF; Adh Fusr KanrRifr This study
E. coli
  DH5α E. coli host strain used for routine cloningStratagene
  INVαF′ E. coli host strain for cloning of PCR productsInvitrogen
  LMG194 E. coli strain for expression of recombinant proteinsInvitrogen
  TX5252INVαF′ (pTEX5252); Ampr Kanr This study
  TX5253DH5α (pTEX5253); Kanr This study
  TX5254LMG194 (pTEX5254); Ampr This study
 pTEX4577Derived from pBluescript SK, used for insertion disruption mutagenesis in enterococci 28
 pBAD/HisAExpression vectorInvitrogen
 pTEX52521,003-bp intragenicace PCR product cloned into pCR2.1 (TA cloning vector)This study
 pTEX52531,100-bpXhoI-KpnI intragenic ace fragment from pTEX5252 cloned into pTEX4577This study
 pTEX52541,008-bp OG1RF ace (coding for complete A domain) cloned into pBAD/HisA expression vectorThis study
  • a Adh+, adherence to CI, CIV, and LN after growth at 46°C; Adh, markedly reduced adherence to CI, CIV, and LN; Ampr, ampicillin resistant; Fusr, fusidic acid resistant; Kanr, kanamycin resistant; Rifr, rifampin resistant.