Table 2.

Nucleotide sequences of primers used for PCR analysis ofstx-flanking regions

PrimerNucleotide sequenceTarget sequenceSource or reference
stxB 1-f5′-ACGCCTGATTGTGTAACTGGA-3′ stxB 1 This study
85a-stx 1-b5′-GACCACCACATCACCTTCTGC-3′orf85aThis study
282-stx 1-b5′-TACCACTGATGAAGGCTGAGG-3′orf282This study
s-stx-b5′-ACCTGTTGTGATTTTTTCCAT-3′Holin S geneThis study
s-stx-b15′-CCAAACAGCAGACTCCCCAG-3′Holin S geneThis study
Q-stx-f5′-CGGAGGGGATTGTTGAAGGC-3′Antiterminator QThis study
215′-CCCGGATCCATGAAAAAAACATTATTAATAGC-3′ stxA 1subunitThis study
2855′-CCTTCGCCACCACATTAACTG-3′ stxA 1subunit 25
5955′-CCGAAGAAAAACCCAGTAACAG-3′ stxA 2subunit 25
5455′-CATGAAGAAGATGTTTATGGCG-3′ stxB 2subunitThis study
4575′-CACCCCGTTCTCATCCGTCATG-3′ stx-flanking regionThis study
4225′-GGTCTTTTGCATCTGTATGG-3′ stx-flanking regionThis study
NinG/5265′-CACAAGCAATGCGTGGTGTGC-3′NinG regionThis study
roi-f15′-CATTGAAATCGCTGAGTTGGT-3′Roi regionThis study
roi-f25′-TCGCAGCACCTAAAGTTGAGT-3′Roi regionThis study
128-15′-AGAATTGGGCGTCATTCACTGGTTG-3′ stxA 2fsubunit 26
stx 2f-s-b5′-GAAGCCCAGAACCAGACTCC-3′Holin S (T4/97)This study
O111fl-15′-GGGCAATGATGACCACAC-3′ stx-flanking regionThis study
O111fl-25′-CAGTCGGTACAGTTTTGAGATT-3′ stx-flanking regionThis study
O111fl-35′-CAATAAAACGCACACCATCC-3′ stx-flanking regionThis study
IS600-f5′-ATGAGCAGAAAAACCCAACG-3′IS600elementThis study