Table 3.

Effect of rIL-18 on IFN-γ and IL-12 production byC. albicans-stimulated cells from naive or vaccinated mice

Cell typearIL-18Cytokine productionb
IFN-γIL-12p70
Naive58 ± 122.04 ± 1.1
+61 ± 101.91 ± 0.8
Vaccinated95 ± 240.81 ± 0.4
+214 ± 31c 4.24 ± 0.7c
  • a Spleen cells were taken from naive or vaccinated caspase 1−/− mice after 3 days of infection with 106 CA-6 cells. Cells were cultured in vitro with heat-inactivated C. albicans and 100 U of rIL-2 per ml in the presence (+) or absence (−) of 50 ng of rIL-18 per ml for 48 h. Vaccinated mice received 106 cells of low-virulence PCA-2, 14 days before reinfection.

  • b Cytokine content (nanograms per milliliter) as assessed by cytokine-specific ELISA.

  • c P < 0.05, rIL-18 (+) versus rIL-18 (−) cultures.