Table 2.

Inhibition of adherence of 46°C-grown E. faecalis OG1RF to ECM proteins by IgGs purified from anti-Ace A (OG1RF-derived) rabbit immune serum

ECM% of cells bound at IgG concn ofa:
0 μg/ml0.001 μg/ml0.01 μg/ml0.1 μg/ml1 μg/ml10 μg/ml100 μg/ml
PIAnti-AcePIAnti-AcePIAnti-AcePIAnti-AcePIAnti-AcePIAnti-Ace
CI24.6 ± 3.8825.3 ± 6.7820.1 ± 4.0427.2 ± 4.9518.0 ± 4.0924.5 ± 5.356.3 ± 1.0421.6 ± 2.862.2 ± 0.3822.0 ± 3.122.3 ± 0.4423.8 ± 3.661.5 ± 0.37
CIV29.6 ± 5.3130.3 ± 6.1526.2 ± 8.3528.8 ± 6.1220.3 ± 3.0528.9 ± 6.617.9 ± 3.4925.9 ± 8.983.2 ± 1.8926.6 ± 5.122.8 ± 0.8126.0 ± 4.732.2 ± 1.25
LN32.0 ± 5.9130.2 ± 1.9426.2 ± 5.1531.4 ± 7.8921.8 ± 1.9729.1 ± 4.678.6 ± 2.0426.4 ± 7.513.5 ± 1.2528.3 ± 4.252.8 ± 0.5632.0 ± 3.032.1 ± 0.49
BSA0.9 ± 0.120.7 ± 0.050.5 ± 0.090.1 ± 0.050.7 ± 0.090.6 ± 0.080.5 ± 0.030.8 ± 0.160.6 ± 0.260.6 ± 0.260.7 ± 0.120.6 ± 0.260.4 ± 0.06
  • a Values are means ± standard deviations for six wells. Results are representative of three independent experiments. PI, IgGs purified from preimmune rabbit serum; Anti-Ace, IgGs purified from polyclonal anti-Ace A rabbit immune serum.