Table 2.

Intracellular survival and multiplication of B. pseudomallei strains

Time (h)bSurvival in cell linea
J774A.1HeLa
844UE16UE5844UE16UE5
4(5.82 ± 1.76) × 104 (3.82 ± 0.18) × 105 (1.30 ± 0.30) × 104 (0.02 ± 0.02) × 102 (0.08 ± 0.02) × 102 (0.02 ± 0.02) × 102
6(7.28 ± 2.22) × 105 (5.62 ± 0.52) × 105 (2.86 ± 1.20) × 105 NDNDND
8(4.68 ± 1.08) × 106 (1.24 ± 0.12) × 106 (1.86 ± 0.02) × 106 NDNDND
12NDNDND(1.20 ± 0.95) × 102 (1.70 ± 1.00) × 102 (0.85 ± 0.20) × 102
24NDNDND(5.11 ± 2.39) × 104 (2.51 ± 2.34) × 105 (3.97 ± 1.92) × 104
  • a Results are mean CFU of intracellular bacteria ± SEM from duplicate wells. ND, not done.

  • b Hours after inoculation of bacteria into host cells.