Table 4.

Lethality of wild-type SPE A and mutants as tested by endotoxin enhancement and miniosmotic pump model

Protein testedNo. dead/total when tested by:
Endotoxin enhancementaMiniosmotic pumpb
Wild type SPE Ac 11/119/11
N20D/C98S0/30/5
N20D/D45N/C98S0/30/5
Q19H/N20D/L41A/L42A/D45N/C98S0/50/5
  • a Animals were intravenously administered 5 μg of wild-type or mutant SPE A per kg. Four hours later, the rabbits were given 10 μg of S. enterica serovar Typhimurium endotoxin intravenously. Animals were monitored for 48 h for lethality.

  • b Miniosmotic pumps contained 500 μg of wild-type or mutant SPE A.

  • c Cumulated total control rabbits.