Table 3.

Inhibition of adherence of 46°C-grown E. faecalis clinical isolates V583 and MC02152 to ECM proteins by IgGs (20 μg/ml) purified from anti-Ace A (OG1RF-derived) rabbit immune serum

ECM% of cells bounda
V583MC02152
No IgGPIAnti-AceNo IgGPIAnti-Ace
CI29.2 ± 1.8728.1 ± 2.843.2 ± 0.1726.0 ± 1.6124.4 ± 1.312.9 ± 0.46
CIV38.1 ± 8.6633.6 ± 6.812.5 ± 0.1727.7 ± 0.3926.5 ± 1.842.3 ± 0.13
LN29.4 ± 1.9627.6 ± 3.532.7 ± 0.7826.7 ± 1.2223.5 ± 0.761.9 ± 0.52
BSA0.7 ± 0.100.6 ± 0.070.6 ± 0.010.6 ± 0.120.5 ± 0.190.6 ± 0.17
  • a Values are mean percentages of cells bound ± standard deviations for six wells. Results are representative of three independent experiments. PI, IgGs purified from preimmune rabbit serum; Anti-Ace, IgGs purified from polyclonal anti-Ace A rabbit immune serum.