Table 3.

β-Gal activity of the gonococcalnorB′-′lacZ fusion

StrainRelevant genotypeβ-Gal activity (Miller units ± SEM)a
Aerobic culturesAnaerobic cultures
+NO2−NO2+NO2−NO2
RUG7021PromoterlesslacZ 0.1 ± 0.010.1 ± 0.010.4 ± 0.110.1 ± 0.03
RUG7500 norB′-′lacZ 23 ± 1.520 ± 1.61,798 ± 18.434 ± 1.9
RUG7501 norB′-′lacZ aniA::ermC 20 ± 1.221 ± 0.542 ± 6.429 ± 0.5
RUG7502 norB′-′lacZ tacP::aniA 164 ± 1119 ± 0.11,094 ± 3341 ± 9.5
RUG7503 norB′-′lacZ narP::ermC 21 ± 0.521 ± 0.41,081 ± 3622 ± 2
  • a Aerobic cultures were grown in GCK broth with shaking in baffled flasks in the presence (+) or absence (−) of 2 mM sodium nitrite (NO2). Anaerobic cultures were grown on plates with (+) or without (−) disks saturated with 20% (wt/vol) sodium nitrite (NO2).