Table 5.

Results of PCR and restriction analysis of Stx2-positiveE. coli strains used in this study with specific primersa

StrainSerotypestx genotypePCR resultb with primer:Sizes (bp) of EcoRI fragments of 545/s-stx-b
Q-stx-f/595 (900 bp)545/457 (1,756 bp)c545/422 (2,403 bp)c545/s-stx-b (3,455 bp)c
EDL933O157:H71, 2++++1,400, 2,100
C600(933W)O157:H72++++1,400, 2,100
A8993O157:H71, 2++++1,400, 2,100
HUSCL8O157:H71, 2++++1,400, 2,100
3574/92O157:H72++++1,400, 2,100
702/88O157:H 2++++1,400, 2,100
1448/97O26:H112++++1,800, 2,100
ED-147O26:H112+++1,800, 2,100
4993/96O26:H112++++1,800, 2,100
6061/96O26:H112++++1,400, 2,100
3639/96O26:H112++++1,400, 2,100
7828/95O103:H21, 2++++1,800, 2,100
2969/99O103:H22++++1,400, 2,100
2791/97O103:H 2++++1,400, 2,100
6366/97O111:H 1, 2++++1,400, 2,100
6833/96O11:H22** +++1,400, 2,100
3985/96O145:H 2* +++1,800, 2,100
893/98O145:H 2* +++1,800, 2,100
5951/97O145:H 2++++1,400, 2,100
6480/96O8:H 2++++1,800, 2,100
RO10O26:H111
  • a For primer description see Table 2.

  • b PCR result: +, product obtained; ∗, NinG positive; ∗∗, NinG-PCR and Roi positive; −, no PCR product obtained.

  • c The PCR product sizes in parentheses were obtained only with E. coli O157 strains. The PCR products of non-O157 strains differed slightly in size.