Table 2.

Recombinant and native CBPs and their roles in virulence

CBPContig no.aLength of open reading frame (bp)No. of CBD repeatsPredicted no. of amino acidsProtein sizeb(kDa)Virulence of mutants defective in production of CBPc
RecombinantNativeNasopharynxSepsis
LytBc42701,47386787670N
LytCc41172,03444916260–65N
CbpDc43741,3474449TN
CbpEc41391,884106287070–75N
CbpFc41031,02383414541NN
CbpGc410342621542230–35
CbpIc4215636521229NN
CbpJc425699973333338NN
  • a Based on the February 1998 National Center for Biotechnology Information database.

  • b Approximated based on migration on an SDS-polyacrylamide gradient gel. T, truncated form was expressed; —, the antibody against rCBP does not recognize the native protein.

  • c N, normal activity; arrow, decreased activity.