Table 1.

Adherence and other characteristics of strains

StrainsAdherenceaEspA secretionbIntiminbFAScGrowthdMCe
Class 1
Class 2
Class 3
  • a Adherence was divided by the mean adherence (100%) of the O157Sakai-T parental strain. ++++, >80%; +++, 80 to 50%; ++, 50 to 25%; +, 25 to 1%; −, <1%. Data are the means of triplicate determinations, which differed by <15%, from representative experiments.

  • b EspA in culture supernatant and intimin in whole bacterial cell were detected by anti-EspA and anti-intimin antibody, respectively. +, detected; −, not detected; +/−, detected at a reduced level.

  • c FAS assays of infected Caco-2 cells were performed by using rhodamine-phalloidin. ND, not determined because nonadherent to Caco-2 cells.

  • d Growth rates of each mutant and the parental wild type were essentially the same. N, normal.

  • e Ability to form microcolonies; +, positive; −, negative.

  • f WT, wild type.