Table 1.

Adherence and other characteristics of strains

StrainsAdherenceaEspA secretionbIntiminbFAScGrowthdMCe
WTf+++++++N+
Class 1
 A3-C10+NDN
 A3-E9+NDN
 A5-E4+NDN
 A6-E7+NDN
 B4-B2+NDN
 B6-H7+NDN
 B8-E7+NDN
 B8-F12+NDN
 B9-F9+NDN
 D5-B12+NDN
Class 2
 A3-H2++/−++N+
 C6-E9+++++N+
 C6-F9++++N+
 C6-H10+++++N+
 C7-A10++++N+
 C8-A2++++N+
 F4-H4++++N+
 F5-F4++++N+
 F6-B9+++++N+
 F6-D11+++++N+
 G2-B12+++++N+
 G4-B6++++N+
 G4-B12++++N+
 G4-C10+++++N+
 G4-E10++++N+
 H7-C4++++N+
Class 3
 G1-E11++N
  • a Adherence was divided by the mean adherence (100%) of the O157Sakai-T parental strain. ++++, >80%; +++, 80 to 50%; ++, 50 to 25%; +, 25 to 1%; −, <1%. Data are the means of triplicate determinations, which differed by <15%, from representative experiments.

  • b EspA in culture supernatant and intimin in whole bacterial cell were detected by anti-EspA and anti-intimin antibody, respectively. +, detected; −, not detected; +/−, detected at a reduced level.

  • c FAS assays of infected Caco-2 cells were performed by using rhodamine-phalloidin. ND, not determined because nonadherent to Caco-2 cells.

  • d Growth rates of each mutant and the parental wild type were essentially the same. N, normal.

  • e Ability to form microcolonies; +, positive; −, negative.

  • f WT, wild type.