Table 1.

Effect of calcium blockers on DAEC C1845-induced apical F-actin disassembly in Caco-2/TC7 cellsa

Cells (treatment)Concn (μM)Mean relative F-actin immunofluorescence intensity ± SEMMean % of cells presenting apical F-actin disassembly ± SEM
Control2.80 ± 0.180
Infected0.32 ± 0.0995 ± 5
Infected (BAPTA/AM)7.51.12 ± 0.17* 60 ± 10*
252.30 ± 0.19* 25 ± 5*
Infected (dantrolene)1000.75 ± 0.20* 75 ± 8*
2002.65 ± 0.35* 15 ± 6*
  • a Apical F-actin distribution was revealed by direct immunofluorescence labeling using phalloidin-fluorescein. Relative F-actin immunofluorescence intensity was reported as the mean ± SEM of arbitrary units measured as described in Materials and Methods. Results are also presented as the mean (± SEM) percentage of cells presenting apical F-actin disorganization relative to the total number of cells observed (54). Dantrolene and BAPTA/AM were present in the incubation medium 60 min before and during infection. Statistical analysis comparing infected cells with infected-treated cells was performed with a Student t test. ∗, significant difference (P < 0.01).