Table 2.

Products encoded by the gene withaor in the vicinity of mini-Tn5Km2

StrainGene productSite (min) onE. coli K-12 genomebAccession no. or reference
A3-H2Hypothetical 17.8-kDa protein (yfjG)59.2P52121 (1)
C6-E9Putative membrane proteinAE000234
C6-F9Putative membrane proteinAE000234
C6-H10Putative membrane proteinAE000234
C7-A10Putative membrane proteinAE000234
C8-A2Hypothetical 8-membrane-spanning 37.6-kDa protein (yiaH)80.2P37669
F4-H4Putative NAGC-like transcriptional regulator (yphHI)57.8AE000341
ABC transporter-periplasmic binding protein YPHF precursorP77269
Hypothetical 127.3-kDa proteinP76585
Serine hydroxy transferase (glyA)38
F5-F4Putative potassium channel (kch)28.3P31069 (32)
F6-B9Hypothetical 10-membrane-spanning 58.4-kDa protein27.2P40877
F6-D11Hypothetical 40.2-kDa protein64.7Q46803
Hypothetical sigma 54-dependent transcriptional regulatorQ46802
G2-B12Putative adherence factor (efa1)AF159462 (34)
G4-B6Arsenite (efflux) pump membrane protein (arsF)78.597
Origopeptidase A (prlC)3
G4-B12Hypothetical 27.7-kDa protein14.6P77627
G4-C10(orf3 of EPEC pathogenicity island LIM)46
G4-E10Lysine-specific permease (lysP)48.543
Hypothetical transcriptional regulatorP32484
H7-C4Hypothetical 10-membrane-spanning 43.6-kDa protein87.5P32135
  • a Products encoded by the gene with mini-Tn5Km2 are in bold type.

  • b The insertion sites placed in the DNA sequences specific to the O157Sakai genome sequence are indicated by a dash (–). The sites of C6-E9, C6-F9, C6-H10, and C7-A10 were in the same ∼3-kb regions encoding an open reading frame partially homologous to the b1372 gene, which encodes a putative membrane protein of 1,122 amino acids of E. coli K-12. F6-D11 was placed in a region containing genes encoding a hypothetical sigma 54-dependent transcriptional regulator and a hypothetical 40.2-kDa protein homologous to ornithine carbamoyltransferase. G4-C10 was placed in orf3 in a region homologous to the EPEC pathogenicity island named LIM, encoding a chaperon-like protein involved in the stable expression of BfpA and intimin (47).