IL-12 and IL-1β production in M. avium-infected DC and Mφa
| Treatment regimen | Time (h) | Mean concn ± SEM (pg/ml) in: | |||
|---|---|---|---|---|---|
| DC of: | Mφ of: | ||||
| IL-12b | IL-1β | IL-12 | IL-1β | ||
| M. avium | 24 | 4,389 ± 210 | 176 ± 22 | 266 ± 16 | 197 ± 39 |
| 48 | 1,136 ± 58 | 194 ± 41 | 214 ± 31 | 181 ± 22 | |
| 72 | 1,202 ± 83 | 136 ± 16 | 94 ± 3 | 148 ± 27 | |
| M. avium + polymyxin B | 24 | 4,031 ± 163 | 186 ± 31 | 284 ± 34 | 187 ± 41 |
| 48 | 2,264 ± 76 | 172 ± 23 | 273 ± 12 | 176 ± 32 | |
| 72 | 1,155 ± 60 | 131 ± 17 | 81 ± 18 | 150 ± 12 | |
↵a As described in Materials and Methods, 5 × 105 DC or Mφ were infected with M. avium in the absence or presence of 10 μg of polymyxin B/ml at an MOI of 10. Concentrations of IL-12 and IL-1β in the culture supernatants collected at the indicated times were measured by enzyme-linked immunosorbent assay. Results are the means ± SEM of two independent experiments. The number of cells in the monolayer did not differ significantly in each experiment. The concentrations of IL-12 and IL-1β in the culture supernatants from noninfected cells were below the sensitivity of the assay (10 pg/ml for IL-12 and 5 pg/ml for IL-1β).
↵b P < 0.05 compared with the amount of IL-12 produced by Mφ at the same time point.