Table 2.

IL-12 and IL-1β production in M. avium-infected DC and Mφa

Treatment regimenTime (h)Mean concn ± SEM (pg/ml) in:
DC of:Mφ of:
IL-12bIL-1βIL-12IL-1β
M. avium 244,389 ± 210176 ± 22266 ± 16197 ± 39
481,136 ± 58194 ± 41214 ± 31181 ± 22
721,202 ± 83136 ± 1694 ± 3148 ± 27
M. avium + polymyxin B244,031 ± 163186 ± 31284 ± 34187 ± 41
482,264 ± 76172 ± 23273 ± 12176 ± 32
721,155 ± 60131 ± 1781 ± 18150 ± 12
  • a As described in Materials and Methods, 5 × 105 DC or Mφ were infected with M. avium in the absence or presence of 10 μg of polymyxin B/ml at an MOI of 10. Concentrations of IL-12 and IL-1β in the culture supernatants collected at the indicated times were measured by enzyme-linked immunosorbent assay. Results are the means ± SEM of two independent experiments. The number of cells in the monolayer did not differ significantly in each experiment. The concentrations of IL-12 and IL-1β in the culture supernatants from noninfected cells were below the sensitivity of the assay (10 pg/ml for IL-12 and 5 pg/ml for IL-1β).

  • b P < 0.05 compared with the amount of IL-12 produced by Mφ at the same time point.