Table 2.

THP-1 sensitivity to FliC is trypsin sensitive

Trypsin treatmentaTNF-α production (pg/106 cells)% Inhibitionb
Expt 1
 No309
 Yes10367
Expt 2
 No155
 Yes5366
Expt 3
 No360
 Yes9773
  • a THP-1 cells (106) were incubated in the presence or absence of 10 μg of trypsin per ml for 20 min at 37°C, washed, and then incubated with 10−9 M FliC for 1 h at 4°C. The cells were then washed and shifted to 37°C for 4 h. The TNF-α production in each culture was measured by ELISA.

  • b Inhibition due to trypsin treatment.