TABLE 2.

Strains and plasmids used in this study

Strains or plasmidRelevant genotype or phenotypeSource or reference
V. cholerae
    CVD110E7946 El Tor Ogawa Δ(cep-ctxB) hlyA::mer::ctxB Hgr 15
    GOA1264Spontaneous Rifr form of CVD110 RifrThis work
    GOA1245GOA1264 res cassette Rifr Kmr SucsThis work
    GOA1246GOA1264 res1 cassette Rifr Kmr SucsThis work
    GOA6WGOA1264 lacZ::pGP704 Apr LacZLaboratory strain
    GOA1745GOA1245 irgA::pGOA1196 Rifr Kmr SucsThis work
    GOA1746GOA1245 irgA::pGOA1197 Rifr Kmr SucsThis work
    GOA1747GOA1246 irgA::pGOA1196 Rifr Kmr SucsThis work
    GOA1748GOA1246 irgA::pGOA1197 Rifr Kmr SucsThis work
    GOA1737GOA1264 ΔVC1500 RifrThis work
    GOA1738GOA1264 ΔVC2487 RifrThis work
    GOA1739GOA1264 ΔVC0488 RifrThis work
    GOA1740GOA1264 ΔVC0622 RifrThis work
    GOA1741GOA1264 ΔVC0874 RifrThis work
    GOA1742GOA1264 ΔVC0130 RifrThis work
    GOA1743GOA1264 ΔVC2705 RifrThis work
    GOA1744GOA1264 ΔVC2646 RifrThis work
E. coli
    SM10λpir thi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pir KmrLaboratory strain
    DH5αλpirF φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR1 supE44 thi-1 gyrA96 relA1::pir 10
Plasmids
    pRR51pBR322::(res-tet-res) 20a
    pGP704 oriR6K mobRP4 Apr 16
    pAC212pGP704::neoLaboratory plasmid
    pCVD442 oriR6K mobRP4 sacB Apr Sucs 5
    pSL134pPCR-Script::(res1-res1)Laboratory plasmid
    pGOA1pSL134lacZ::(res1-neo-sacB-res1)This work
    pGOA2pPCR-Script::(res)This work
    pGOA3pPCR-Script::(res1)This work
    pGOA4pPCR-Script::(res-res)This work
    pGOA5pPCR-Script::(res1-res1)This work
    pGOA6pGOA4::neo-sacBThis work
    pGOA7pGOA5::neo-sacBThis work
    pSL111pGP704::lacZLaboratory plasmid
    pIVET5 oriR6K mobRP4 lacZ tnpR Apr 2
    pIVET5npIVET5 derivativeThis work
    pRESpSL111::(res-neo-sacB-res)This work
    pRES1pSL111::(res1-neo-sacB-res1)This work
    pGOA1193pIVET5n tnpRThis work
    pGOA1194pIVET5n tnpRmut168This work
    pGOA1195pIVET5n tnpRmut135This work
    pGOA1196pGOA1193::PirgAThis work
    pGOA1197pGOA1195::PirgAThis work