TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesaSource or reference
Strains
    E. coli
        DH5αsupE44 Δlac U169 (Φ80 lacZΔM15)R17 recA1 endA1 gyrA96 thi-1 relA1Gibco-BRL
        EC1000Kmr; MC1000 derivative carrying a single copy of pWV01 repA in glgB46
        EC101E. coli JM101 with repA from pWV01 integrated in the chromosome35
    L. monocytogenes
        LO28Serotype 1/2cP. Cossart
        ΔbshLO28 derivative with bsh deletedThis study
        ΔpvaLO28 derivative with pva deletedThis study
        ΔbtlBLO28 derivative with btlB deletedThis study
        Δbsh ΔpvaLO28 derivative with bsh and pva deletedThis study
        Δbsh Δpva ΔbtlBLO28 derivative with bsh, pva, and btlB deletedThis study
        Δbsh pPL2::bshΔbsh with bsh integrated at tRNAArg-attBThis study
        Δpva pPL2::pvaΔpva with pva integrated at tRNAArg-attBThis study
        ΔbtlB pPL2::btlBΔbtlB with btlB integrated at tRNAArg-attBThis study
        pORI19::prfALO28 derivative with an insertion in prfAThis study
        pORI19::sigBLO28 derivative with an insertion in sigBThis study
        EGDeSerotype 1/2aW. Goebel
        EGDeΔpvaEGDe derivative with pva deletedThis study
        EGDeΔbtlBEGDe derivative with btlB deletedThis study
        EGDe pORI19::bshEGDe derivative with an insertion in bshThis study
        EGDe pORI19::prfAEGDe derivative with an insertion in prfAThis study
        EGDe pORI19::sigBEGDe derivative with an insertion in sigBThis study
Plasmids
    pKSV7Cmr; temperature sensitive49
    pORI13Emr; promoterless lacZ, Ori+, RepA derivative of pWV0146
    pORI19Emr; Ori+, RepA derivative of pORI2835
    pVE6007Cmr; temperature-sensitive derivative of pWV0137
    pPL2Cmr; integrates at the PSA phage attachment site within a tRNAArg gene on the chromosome34
  • a Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Kmr, kanamycin resistant.