Table 1.

Strains and plasmids used in this study

Strain or plasmidDetailsReference or sourcea
Strains
 E2348/69Wild-type EPEC O127:H6 29
 EDL933Wild-type EHEC O157:H7 37
 85-170EHEC O157:H7 Δstx 45
 86-24Wild-type EHEC O157:H7 20
 SE941O55:H7 strainCVD diarrheal isolate
 SE796EPEC E2348/69 Δler::kan This study
 SE1099EHEC 85-170 Δler::kan This study
 SE1101EHEC 86-24 Δler::kan This study
 SE860SE796(pCVD456)This study
 SE1104SE796(pSE1100)This study
 SE1110SE796(pSE1092)This study
 SE1140SE1099(pSE1092)This study
 TE2680MC4100recD::Tn10 12
Plasmids
 pCVD456pMOB::ler orf2345EPEC 31
 pSE1092pACYC184::lerEHEC This study
 pSE1093pBR322::lerEHEC This study
 pSE1100pBR322::lerEPEC This study
 pTHK113 hns 25
 pJG9 ori ts cat sacB J. Galen, unpublished data
 pRS551 lacZ reporter gene fusion vector 41
  • a CVD, Center for Vaccine Development.