Table 2.

Primers used for construction of each nonpolar deletion

Deleted genePrimerPrimer sequence (5′ to 3′)
pmrHJG234CAT GCC ATG GCA TTG AAA GCC GCT TTT C
JG235GCT CTA GAC CCG TGC TGT CTG ACA GG
JG236CAT GCC ATG GGA TAG CAG GAC AAT AAG CAT G
JG237CGG AAT TCG TGA TGC ACC GGG ATC TC
pmrFJG183CAT GCC ATG GGT CAA ACA TGC TTA TTG TCC TGC TAT C
JG181GCT CTA GAC CCT TGA CCG TAA TCC CCG TG
JG184CAT GCC ATG GGC CGT TTA CTG AGG AAA GCC ACC AAT G
JG202CGG AAT TCG CCG TTA TAG CTG AAC GCG CC
pmrIJG185CAT GCC ATG GTG GTG GCT TTC CTC AGT AAA C
JG187GCT CTA GAC AGG ACA GCC TGT TTC GCA AAT CG
JG186CAT GCC ATG GCG ATA TCG CGG AAC GCG CAT C
JG188CGG AAT TCC CAG GTA TTG AAC GAC TGC GE
pmrJJG245CAT GCC ATG GGC GTA AAC CGA CTT TCG TCA TGA TGC G
JG246GCT CTA GAC AGC GGT AAA CGT CGC ATT CG
JG247CAT GCC ATG GGG CAG CGA GCG CCT CAT GAT G
JG248CGG AAT TCG AAA GAA CAT AGG TGG GCA GTT TC
pmrKJG191CAT GCC ATG GGC GTA TCG ATT TCA TCA TGA G
JG193GCT CTA GAC ACC ATG CGT GGC AGA CTC AC
JG192CAT GCC ATG GGG TGT TAA TTC AGT ATC GGC C
JG196CGG AAT TCG CAA GCC ATC ATG ATC TGG CG
pmrLJG194CAT GCC ATG GCA GAA CGA CGC CGA TCA TTT AGG C
JG193GCT CTA GAC ACC ATG CGT GGC AGA CTC AC
JG195CAT GCC ATG GGG CAT AAT GGG CGT AAT GTG G
JG196CGG AAT TCG CAA GCC ATC ATG ATC TGG CG