Table 1.

Bacterial strains and plasmids and relevant properties

Strain or plasmidGenotype or relevant phenotypeSource or reference
Strainsa
 SM10λpir thi-1 thr-1 leuB6 supE44 tonA21 lacY1 recA::RP4-2-Tc::Mu
 JSG210ATCC 14028s (WT)ATCC
 JSG430 pho24 pmrA::Tn10d (PhoPc PmrA)9
 JSG437 phoP::Tn10d-tet pmrA505 zjd::Tn10d-cam (PhoPPmrAc)9
 JSG435ATCC 14028s pmrA 505 zjd::Tn10d-cam (PmrAc)9
 JSG421 pmrA::Tn10d(PmrA)9
 JSG485JSG435pmrF1::Tn10d 8
 JM109F′traD36 lacI Δ(lacZ)M15proA+B+/e14 (mcrA) Δ(lac-proAB) thi gyrA96 (Nalr)endA1 hsdR17 (rK mK ) relA1 supE44 recA1 Promega
 JSG224 phoN2 zxx::6251 Tn10d-cam (CS019)15
 JSG844JSG435rpsL (PmrAc Smr)This study
 JSG1097JSG844; ΔpmrH This study
 JSG911JSG844; ΔpmrF This study
 JSG868JSG844; ΔpmrI This study
 JSG1098JSG844; ΔpmrJ This study
 JSG883JSG844; ΔpmrK This study
 JSG884JSG844; ΔpmrL This study
 JSG1046JSG421 pmrM::luc This study
 JSG1047JSG435 pmrM::luc This study
 JSG1048JSG485 pmrM::luc This study
 JSG989JSG224 pmrD::luc This study
Plasmids
 pGPL01Firefly luciferase recorder, suicide vector9
 pKAS32 rpsL suicide vector19
 pSR01pKAS32; ΔpmrH This study
 pSR02pKAS32; ΔpmrI This study
 pSR03pKAS32; ΔpmrK This study
 pSR04pKAS32; ΔpmrL This study
 pSR05pKAS32; ΔpmrF This study
 pSR06pKAS32; ΔpmrJ This study
 pLB02Firefly luciferase recorder, suicide vector7
 pGPLM02pGPL01 with pmrM EcoRI-KpnI PCR fragment amplified with JG252 and JG253This study
 pMRD1pGPL01 with pmrD EcoRI-KpnI PCR fragment amplified with JG177 and JG178This study
 pLB2223118-bp pmrHFIJKLM operon promoter-fragment (JG122 and JG123) in pLB02 (EcoRI-KpnI)This study
 pQPA01pQE30 containing the pmrA (WT) gene amplified with primers JG106 and JG107 (BamHI-PstI)This study
 pQP505pQE30 containing the pmrA-constitutive (JSG435) gene amplified with primers JG106 and JG107 (BamHI-PstI)This study
 pQE30Amino-terminal His tag vectorQiagen
  • a Strain SMI0λpir is E. coli; the other strains are S. enterica serovar Typhimurium.