TABLE 1.

Strains and plasmids

Strain or plasmidCommentReference or source
S. aureus
    RN4220Mutant strain of 8325-4 that accepts foreign DNA39
    RN6390agr+ laboratory strain related to 8325-4, maintains hemolytic pattern when propagated on sheep erythrocytes39
    COLMethicillin-resistant laboratory strain54
    NewmanLaboratory strain37
    FDA486Wild-type strain FDA486, sigB+, intact rsbU43
    RN6911agr mutant of RN6390 (Δagr::tetM)42
    ALC3043sarT mutant of RN6390 (ΔsarT::tetK)This study
    ALC2530mgrA mutant of RN6390 (ΔmgrA::ermC)21
    ALC2531ALC2530 complemented with the mgrA gene in single copy21
    ALC2542mgrA mutant of COL (ΔmgrA::ermC)This study
    ALC2547mgrA mutant of Newman (ΔmgrA::ermC)This study
    ALC3632mgrA mutant of FDA486 (ΔmgrA::ermC)This study
    ALC2537agr mgrA (deletion) double mutant of RN639021
    ALC3046sarT mgrA (deletion) double mutant of RN6390This study
    ALC3188agr sarT (deletion) double mutantThis study
    ALC3191mgrA sarT agr (deletion) triple mutantThis study
E. coli
    XL-1 BlueGeneral-purpose host strain for cloning31
    InvαF′Host strain for the TA cloning vector (pCR2.1)Invitrogen
Plasmids
    pCR2.1E. coli PCR cloning vectorInvitrogen
    pALC1484Derivative of pSK236 containing the promoterless gfpuvr gene25
    pALC1740pALC1484 with hla promoter fragment27
    pALC1741pALC1484 with spa promoter fragment27
    pALC1743pALC1484 with P3 agr promoter fragment25
    pALC3179pALC1484 with sarS promoter fragmentThis study
    pALC1594235-bp hla promoter region in pCR2.113
    pALC2321sarS promoter region in pCR2.146
    pALC1996agr P2-P3 promoter region in pALC148414
    pRN6735Contains promoterless RNAIII under control of the blaZ promoter26