TABLE 1.

Observations from GMS- and immunohistochemically stained C. albicans-inoculated mouse tissue sectionsa

Inoculation strainTime point (h)Resultb
KidneyHeartLiver GMSSpleen GMS
GMSIHCGMSIHC
Uninfected48
CAI1212+/+−/−+/+−/−−/+−/−
24+/+−/−+/−−/−+/−−/−
48+/+−/−+/+−/−+/+−/−
PTPII-GFP12+/++/+−/−−/−−/+−/+
24+/++/+−/−−/−+/++/−
48+/++/+−/+−/+−/−−/+
PALS1-GFP12+/++/+−/+−/+−/−−/−
24+/++/++/++/++/+−/+
48+/++/++/++/+−/−−/−
PALS3-GFP12+/++/++/++/+−/−−/−
24+/++/+−/+−/+−/−−/−
48+/++/+−/−−/−−/−+/+
PALS5-GFP12+/+−/−+/−−/−+/−+/−
24+/+−/−+/+−/−+/−−/−
48+/+−/−−/+−/−−/+−/−
PALS6-GFP12−/−−/−−/−−/−−/−−/−
24+/+−/−−/+−/−−/−−/−
48+/+−/−−/−−/−−/−−/+
PALS7-GFP12+/+−/−+/+−/−−/+−/−
24+/+−/−+/+−/−+/−−/−
48+/+−/−+/+−/−+/+−/−
PALS9-GFP12+/+−/−−/−−/−−/−−/−
24+/+−/−−/−−/−−/−−/−
48+/+−/−−/−−/−+/−+/−
  • a Tissues from PALS-GFP-infected mice were analyzed using Gomori methenamine silver (GMS) staining and anti-GFP immunohistochemical (IHC) detection. Each entry in the table shows results from a pair of mice at each time point.

  • b +, presence of fungal element(s) stained black for GMS or brown for IHC visible at ×400 magnification. −, absence of fungal element(s) stained by GMS or lack of staining by IHC in the presence of GMS-positive fungal element(s). All lung sections were negative for GMS and IHC staining at all time points in all animals. All liver and spleen sections were negative by IHC staining, so only GMS results are reported in the table.