TABLE 1.

Oligonucleotide primers and LightCycler hybridization probes

Target geneGenBank accession no.PrimerSequencea
S. aureus icaAAF086783icaUTGGCTGTATTAAGCGAAGTC
icaLTTCCAAAGACCTCCCAATGT
icaFLTGTCGACGTTGGCTACTGGGAT-FL
icaLCRed640-CTGATATGATTACCGAAGATATTGCAGT-ph
S. aureus gyrD10489gyrUTTATGGTGCTGGGCAAATACA
gyrLCACCATGTAAACCACCAGATA
gyrFL1ATTTTAACTGTTTTACATGCTGGTGGTAA-FL
gyrLC1Red640-TTTGGCGGTGGCGGATACA-ph
S. epidermidis icaAU43366Epi-icaUCAATCTCTTGCAGGAGC
Epi-icaLCTTGAGCCCATCGAACC
Epi-icaFL1CAAAGATGTAGGTTATTGGGATACTGACA-FL
Epi-icaLC1Red640-ATTACTGAGGATATTGCTGTTTCATGGA-ph
S. epidermidis gyrAF314404Epi-gyrUGGTATGTATATTGGTTCAACTTCAG
Epi-gyrLGTGAAGACCGCCAGAT
Epi-gyrFL2GGGTTGCACCATTTAGTATGGGAAA-FL
Epi-gyrLC2Red640-TGTTGATAATAGTATTGACGAGGCATTAGC-ph
  • a FL, fluorescein; Red640, LightCycler Red640-N-succinimide ester; ph, 3′-phosphate.